PRESENCE AND EXPRESSION OF HUMAN PAPILLOMAVIRUS SEQUENCES IN HUMAN CERVICAL-CARCINOMA CELL-LINES

  • 1 January 1985
    • journal article
    • research article
    • Vol. 119  (3) , 361-366
Abstract
A series of human carcinoma cell lines was examined for human papillomavirus (HPV) DNA sequences with the use of HPV-6, HPV-11, HPV-16 and HPV-18 DNA probes. Of 8 cell lines derived from human cervical carcinomas 6 were shown to contain integrated HPV DNA sequences. In 5 of these 6 lines, HPV-specific polyadenylated RNA species could also be identified. The expression of HPV sequences was detected in 3 lines with a HPV-18 DNA probe and in 2 lines with a HPV-16 DNA probe. On the 2 lines which contained HPV-16 specific RNA, 1 contained HPV DNA sequences which hybridized only to an HPV-16 probe, and the other contained HPV DNA sequences which hybridized to both HPV-16 and HPV-18 DNA probes. Six cell lines established from human squamous-cell carcinomas of the bladder, pharynx, lung, esophagus and vulva were negative for HPV-6, HPV-11, HPV-16 and HPV-18 DNA sequences under stringent hybridization conditions.