INSEMINATION OF THE GUINEA-PIG BY INTRAPERITONEAL INJECTION

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Abstract
Intraperitoneal injection has been shown to be a method of insemination which produces a high conception rate in guinea-pigs. The proportion that become pregnant increases from 0 to 100% by raising the number of spermatozoa inseminated over the comparatively narrow range of 3·0 × 107-5·0 × 107. The conception rate is high in animals inseminated between 0 and 16 hr after the end of oestrus; all animals (17) inseminated with more than 5·0 × 107 spermatozoa during the first half of this period became pregnant. The incidence of pregnancy is very low in animals inseminated intraperitoneally during oestrus and at 18 hr or more after oestrus. The temporal relation between successful insemination and ovulation is the reverse of that obtaining in mated animals or after intravaginal insemination [Blandau & Young, 1939]. Fertility, determined by an estimate of the fertilization rate, by the number of foetuses in utero and the rate of foetal development is similar to that following mating. Reasons for the failure of fertilization in animals intraperitoneally inseminated during oestrus are discussed.

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