Escherichia coli mutY-dependent mismatch repair involves DNA polymerase I and a short repair tract
- 1 July 1994
- journal article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 244 (4) , 444-450
- https://doi.org/10.1007/bf00286698
Abstract
Repair of heteroduplex DNA containing an A/G mismatch in a mutL background requires the Escherichia coli mutY gene function. The mutY-dependent in vitro repair of A/G mismatches is accompanied by repair DNA synthesis on the DNA strand bearing mispaired adenines. The size of the mufY-dependent repair tract was measured by the specific incorporation of α-[32P]dCTP into different restriction fragments of the repaired DNA. The repair tract is shorter than 12 nucleotides and longer than 5 nucleotides and is localized to the 3′ side of the mismatched adenine. This repair synthesis is carried out by DNA polymerase I.Keywords
This publication has 35 references indexed in Scilit:
- The vsr gene product of E. coli K-12 is a strand- and sequence-specific DNA mismatch endonucleaseNature, 1991
- Escherichia coli mutY gene encodes an adenine glycosylase active on G-A mispairs.Proceedings of the National Academy of Sciences, 1989
- DNA Mismatch Correction in a Defined SystemScience, 1989
- Escherichia coli mutY gene product is required for specific A-G----C.G mismatch correction.Proceedings of the National Academy of Sciences, 1988
- d(GATC) sequences influenceEscherichia colimismatch repair in a distance-dependent manner from positions both upstream and downstream of the mismatchNucleic Acids Research, 1988
- Escherichia coli endonuclease III is not an endonuclease but a β-elimination catalystBiochemical Journal, 1987
- Requirement for d(GATC) sequences in Escherichia coli mutHLS mismatch correction.Proceedings of the National Academy of Sciences, 1987
- Characterization of the Escherichia coli x-ray endonuclease, endonuclease IIIBiochemistry, 1983
- Asymmetric repair of bacteriophage T7 heteroduplex DNAMolecular Genetics and Genomics, 1981
- DNA N-glycosylases and UV repairNature, 1980