Phosphatidylinositol(4,5)bisphosphate and Phosphatidylinositol(4)phosphate in Plant Tissues
- 1 March 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 89 (3) , 888-892
- https://doi.org/10.1104/pp.89.3.888
Abstract
Pea (Pisum sativum) leaf discs or swimming suspensions of Chlamydomonas eugametos were radiolabeled with [3H]myo-inositol or [32P]Pi and the lipids were extracted, deacylated, and their glycerol moieties removed. The resulting inositol trisphosphate and bisphosphate fractions were examined by periodate degradation, reduction and dephosphorylation, or by incubation with human red cell membranes. Their likely structures were identified as d-myo-inositol(1,4,5)trisphosphate and d-myo-inositol(1,4,)-bisphosphate. It is concluded that plants contain phosphatidylinositol(4)phosphate and phosphatidylinositol(4,5)bisphosphate; no other polyphosphoinositides were detected.This publication has 27 references indexed in Scilit:
- Type I phosphatidylinositol kinase makes a novel inositol phospholipid, phosphatidylinositol-3-phosphateNature, 1988
- Phosphatidylinositol 4,5-bisphosphate phosphodiesterase in higher plantsBiochemical Journal, 1988
- l-myo-inositol 1,4,5,6-tetrakisphosphate is present in both mammalian and avian cellsBiochemical Journal, 1988
- Auxin induces rapid changes in phosphatidylinositol metabolitesNature, 1988
- A rapid separation method for inositol phosphates and their isomersBiochemical Journal, 1987
- Inositol(1,3,4,5)tetrakisphosphate-induced activation of sea urchin eggs requires the presence of inositol trisphosphateBiochemical and Biophysical Research Communications, 1987
- Inositol(3,4)bisphosphate and inositol(1,3)bisphosphate in GH4 cells - evidence for complex breakdown of inositol(1,3,4)trisphosphateBiochemical and Biophysical Research Communications, 1987
- 1H, 13C and 31P n.m.r. spectra and molecular conformation of myo-inositol 1,4,5-triphosphateBiochemical Journal, 1986
- Release of Ca2+ from plant hypocotyl microsomes by inositol-1,4,5-trisphosphateBiochemical and Biophysical Research Communications, 1985
- Metabolism of inositol 1,4,5-trisphosphate and inositol 1,3,4-trisphosphate in rat parotid glandsBiochemical Journal, 1985