Separation of Progonadotropic and Antigonadotropic Activities in Ovine and Equine HCG Antisera

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Abstract
The antigonadotropic factor (antibodies) was separated from the progonadotropic factors (probodies) in ovine and equine HCG antisera and in a gamma globulin fraction of equine HCG antiserum by passing the sera, or a serum salt fractionation product through a column in which HCG had been covalently coupled to activated Sepharose. The probodies passed through the column whereas the HCG antibodies were bound to the HCG on the column.

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