Survival of Salmonella enterica Serovar Newport in Manure and Manure-Amended Soils
- 1 September 2006
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 72 (9) , 5777-5783
- https://doi.org/10.1128/aem.00791-06
Abstract
Salmonella enterica serovar Newport has undergone a rapid epidemic spread in dairy cattle. This provides an efficient mechanism for pathogen amplification and dissemination into the environment through manure spreading on agricultural land. The objective of this study was to determine the survival characteristics of Salmonella serovar Newport in manure and manure-amended soils where the pathogen may be amplified. A multidrug-resistant (MDR) Salmonella serovar Newport strain and a drug-susceptible (DS) strain, both bovine isolates, were inoculated into dairy manure that was incubated under constant temperature and moisture conditions alone or after being mixed with sterilized or nonsterilized soil. Salmonella serovar Newport concentrations increased by up to 400% in the first 1 to 3 days following inoculation, and a trend of steady decline followed. With manure treatment, a sharp decline in cell concentration occurred after day 35, possibly due to microbial antagonism. For all treatments, decreases in Salmonella serovar Newport concentrations over time fit a first-order kinetic model. Log reduction time was 14 to 32 days for 1 log10, 28 to 64 days for 2 log10, and 42 to 96 days for 3 log10 declines in the organisms9 populations from initially inoculated concentrations. Most-probable-number monitoring data indicated that the organisms persisted for 184, 332, and 405 days in manure, manure-amended nonsterilized soil, and manure-amended sterilized soil, respectively. The MDR strain and the DS strain had similar survival patterns.Keywords
This publication has 27 references indexed in Scilit:
- Detection of a bla SHV Extended-Spectrum β-Lactamase in Salmonella enterica Serovar Newport MDR-AmpCJournal of Clinical Microbiology, 2005
- Possible Animal Origin of Human-Associated, Multidrug-Resistant, Uropathogenic Escherichia coliClinical Infectious Diseases, 2005
- Use of Antibiotic Susceptibility Patterns and Pulsed-Field Gel Electrophoresis To Compare Historic and Contemporary Isolates of Multi-Drug-Resistant Salmonella enterica subsp. enterica Serovar NewportApplied and Environmental Microbiology, 2004
- Molecular Characterization of Cephalosporin-Resistant Salmonella enterica Serotype Newport Isolates from Animals in PennsylvaniaJournal of Clinical Microbiology, 2002
- Salmonella enterica Serovar Typhimurium and Escherichia coli Contamination of Root and Leaf Vegetables Grown in Soils with Incorporated Bovine ManureApplied and Environmental Microbiology, 2002
- Fate of Escherichia coli O157:H7 in Manure-Amended SoilApplied and Environmental Microbiology, 2002
- Persistence of a Salmonella enterica Serovar Typhimurium DT12 Clone in a Piggery and in Agricultural Soil Amended with Salmonella -Contaminated SlurryApplied and Environmental Microbiology, 2001
- Animal and Human Multidrug-Resistant, Cephalosporin-Resistant Salmonella Isolates Expressing a Plasmid-Mediated CMY-2 AmpC β-LactamaseAntimicrobial Agents and Chemotherapy, 2000
- Survival ofEscherichia coliO157:H7 andSalmonella typhimuriumin cow manure and cow manure slurryFEMS Microbiology Letters, 1999
- Destruction ofSalmonella typhimurium, Escherichia coliO157:H7 andListeria monocytogenesin chicken manure by drying and/or gassing with ammoniaFEMS Microbiology Letters, 1999