Isolation of the nucleic acid of Newcastle disease virus (NDV).
- 1 September 1965
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 54 (3) , 794-800
- https://doi.org/10.1073/pnas.54.3.794
Abstract
Newcastle disease virus (NDV) has been purified from chorioallantoic fluid with little loss of infectivity. Intact nucleic acid isolated using SDS and phenol extraction consists of single-stranded RNA. Two distinct RNA components are always recovered. One component is thought to be intact viral RNA with S20, w=57 in 0.10 [image] salt and an estimated molecular weight of 7.5x106 . One such RNA molecule per virus particle would account for all of the RNA found in purified virus preparations. The other component which sediments at approximately 3S and is heterogeneous is thought to consist of degraded viral RNA.This publication has 19 references indexed in Scilit:
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