0KT3‐Induced Cytokine mRNA Expression in Human Peripheral Blood Mononuclear Cells Measured by Polymerase Chain Reaction

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Abstract
The kinetic profile of cytokine gene expression in normal human peripheral mononuclear cells (MNC) activated by an anti-CD3 monoclonal antibody was studied. The presence or absence of 10 different cytokine mRNA were measured in a polymerase chain reaction (PCR) assisted mRNA amplification assay. Afler 2 h of stimulation the mRNA for interleukin-lα (IL-lα). interleukin-2 (IL-2). interleukin-3 (IL-3). tumour necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) were detectable and remained present during the whole time period studied (22 h). lnterleukin-6 (IL-6) and granulocyte macrophage colony stimulating factor (GM-CSF) were detected after 4 h. while interleukin-1O (IL-1O) mRNA did not appear until after 7 h: they all remained expressed at 22 h. A transient expression of interleukin-4 (IL-4) mRNA was observed between 4 and 7 h of stimulation. No gene expression of granulocyte colony stimulating factor (G-CSF) was detected at any time. These results show that anti-CD3 stimulation of MNC leads to a rapid sequential induction of different cytokine mRNA. some with a very transient expression.