A novel effect of EGF on mRNA stability

1 January 1988

journal article
research article
Published by Oxford University Press (OUP) in Nucleic Acids Research

Vol. 16 (11) , 4957-4986
https://doi.org/10.1093/nar/16.11.4957

Abstract

Expression of the epidermal growth factor (EGF) receptor gene is stimulated by EGF and the phorbol ester, 48-phorbol 12-myristate 13-acetate (PMA). PMA elevates EGF receptor mRNA levels in human KB epidermoid carcinoma cells, but does not significantly affect the half-life of this mRNA when its decay is examined after the addition of actinomycin D. In contrast, EGF greatly prolongs the half-life of EGF receptor mRNA suggesting a possible mechanism for the stimulatory effect of EGF on EGF receptor mRNA levels. EGF also stabilizes .beta.-tubulin and .beta.-actin mRNAs but has very little effect on the degradation of total mRNA.

Keywords

This publication has 23 references indexed in Scilit:

Induction of c-fos gene and protein by growth factors precedes activation of c-myc
Nature, 1984
Specific stimulation of actin gene transcription by epidermal growth factor and cycloheximide.
Proceedings of the National Academy of Sciences, 1984
Extreme instability of myc mRNA in normal and transformed human cells.
Proceedings of the National Academy of Sciences, 1984
Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter
Nucleic Acids Research, 1984
Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed.
Molecular and Cellular Biology, 1983
Epidermal growth factor rapidly stimulates prolactin gene transcription
Nature, 1982
Unpolymerized tubulin modulates the level of tubulin mRNAs
Cell, 1981
Detection, sizing, and quantitation of polyadenylated ribonucleic acid in the nanogram-picogram range
Biochemistry, 1980
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease
Biochemistry, 1979
Epidermal Growth Factor
1979