Studies on Polychrome Methylene Blue I. Eosinates, their Spectra and Staining Capacity

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Abstract
The absorption spectra of eosinates of thiazin dyes in water exhibit absorption maxima at the same spectral locations as do the individual component dyes in aqueous soln. Commercial samples of Wright''s stain showing thiazin ab- sorption maxima between. 620 and 660 millimicrons generally give satisfactory blood stains. Nuclear staining is redder and cytoplasm grayer blue in 620-640 range, and conse-quently staining of malaria parasites ia less satisfactory in that range. The best malaria stains show their thiazin absorption maxima usually between 650 and 660 millimicrons. Successive batches of Wright''s stain made by the same manufacturer, as well as experimental laboratory lots, may show wide variations in their thiazin absorption maxima and in their staining characteristics.