Biosynthesis of renal protein in aminonucleoside nephrosis

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Abstract
The incorporation of labelled amino acids into microsomal and soluble protein was measured in homogenate fractions of liver and kidney cortex of control and aminonucleoside nephrotic rats. In both the control and the nephrotic rat preparations, the incorporation was higher in liver than in kidney homogenate fractions. In nephrotic rat preparations compared to control rat preparations, there was a large increase in the incorporation of labelled amino acids into kidney protein and a smaller increase in the incorporation into liver protein. This increase in the incorporation of amino acids was found in the microsomal fraction rather than in the soluble fraction, but it depended upon alterations in both the microsomal and soluble fractions obtained from nephrotic kidney. The increase could not be explained by increased endogenous amino acids in nephrotic preparations. A differential inhibitory effect of high levels of guanosine triphosphate on the incorporation of L-leucine-U-14C into ribosomal protein in ceil-free systems was observed and was more pronounced in ribosomal preparations from kidney of aminonucleoside nephrotic rats.

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