EFFECT OF CYCLIC-AMP ON TRANSFORMATION AND PROLIFERATION OF LEISHMANIA CELLS

  • 1 January 1978
    • journal article
    • research article
    • Vol. 29  (4) , 439-442
Abstract
A correlation between cyclic[c]AMP concentration within Leishmania cells and proliferation and transformation is demonstrated. By addition of dibutyryl cAMP and cAMP-phosphodiesterase inhibitors to the culture medium the intracellular level of cAMP was increased. In the presence of 1 mM caffeine the level of cAMP accumulated in L. tropica promastigotes from 90 picomoles up to 380 picomoles per 109 cells, whereas the proliferation rate decreased to 50%. In L. donovani the transformation of amastigotes to promastigotes was inhibited by addition of dibutyryl cAMP and cAMP and caffeine and papaverine. Especially caffeine (2 mM) and papaverine (0.1 mM) reduced the transformation rate to < 5% after 48 h, compared to 35% of the control.

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