Design and use of conditional MHC class I ligands
Top Cited Papers
- 5 February 2006
- journal article
- Published by Springer Nature in Nature Medicine
- Vol. 12 (2) , 246-251
- https://doi.org/10.1038/nm1360
Abstract
Major histocompatibility complex (MHC) class I molecules associate with a variety of peptide ligands during biosynthesis and present these ligands on the cell surface for recognition by cytotoxic T cells. We have designed conditional MHC ligands that form stable complexes with MHC molecules but degrade on command, by exposure to a defined photostimulus. 'Empty MHC molecules' generated in this manner can be loaded with arrays of peptide ligands to determine MHC binding properties and to monitor antigen-specific T-cell responses in a high-throughput manner. We document the value of this approach by identifying cytotoxic T-cell epitopes within the H5N1 influenza A/Vietnam/1194/04 genome.Keywords
This publication has 30 references indexed in Scilit:
- HLA-Ig-based artificial antigen-presenting cells: setting the terms of engagementClinical Immunology, 2004
- Detection and Characterizationof Cellular Immune Responses Using Peptide–MHC MicroarraysPLoS Biology, 2003
- Cancer Regression and Autoimmunity in Patients After Clonal Repopulation with Antitumor LymphocytesScience, 2002
- H-2 class I knockout, HLA-A2.1-transgenic mice: a versatile animal model for preclinical evaluation of antitumor immunotherapeutic strategiesEuropean Journal of Immunology, 1999
- Phenotypic Analysis of Antigen-Specific T LymphocytesScience, 1996
- Importance of Peptide Amino and Carboxyl Termini to the Stability of MHC Class I MoleculesScience, 1994
- Crystal Structures of Two Viral Peptides in Complex with Murine MHC Class I H-2K bScience, 1992
- Allele-specific motifs revealed by sequencing of self-peptides eluted from MHC moleculesNature, 1991
- Empty MHC class I molecules come out in the coldNature, 1990
- Time-resolved X-ray crystallographic study of the conformational change in Ha-Ras p21 protein on GTP hydrolysisNature, 1990