Erythrocyte structure and dynamics quantified by Hilbert phase microscopy
- 1 January 2005
- journal article
- Published by SPIE-Intl Soc Optical Eng in Journal of Biomedical Optics
- Vol. 10 (6) , 060503-060503-3
- https://doi.org/10.1117/1.2149847
Abstract
We present a new quantitative method for investigating red blood cell morphology and dynamics. The instrument integrates quantitative phase microscopy with an inverted microscope, which makes it particularly suitable for the noninvasive assessment of live erythrocytes. In particular, we demonstrate the ability of this approach to quantify noninvasively cell volume and dynamic morphology. The subnanometer path-length sensitivity at the millisecond time scales is exemplified by measuring the hemoglobin flow out of the cell during hemolysis.Keywords
This publication has 14 references indexed in Scilit:
- Hilbert phase microscopy for investigating fast dynamics in transparent systemsOptics Letters, 2005
- Peroxynitrite induces senescence and apoptosis of red blood cells through the activation of aspartyl and cysteinyl proteasesThe FASEB Journal, 2004
- Membrane Undulations Driven by Force Fluctuations of Active ProteinsPhysical Review Letters, 2004
- Fourier phase microscopy for investigation of biological structures and dynamicsOptics Letters, 2004
- Imaging erythrocytes under physiological conditions by atomic force microscopyBiochimica et Biophysica Acta (BBA) - Biomembranes, 2001
- Noninterferometric Phase Imaging with Partially Coherent LightPhysical Review Letters, 1998
- Correlation between local cell membrane displacements and filterability of human red blood cellsFEBS Letters, 1992
- Shape fluctuations of polymerized or solidlike membranesPhysical Review Letters, 1990
- Dynamic reflection interference contrast (RIC-) microscopy : a new method to study surface excitations of cells and to measure membrane bending elastic moduliJournal de Physique, 1987
- Frequency spectrum of the flicker phenomenon in erythrocytesJournal de Physique, 1975