Sequence-Selective Recognition of DNA by Strand Displacement with a Thymine-Substituted Polyamide
- 6 December 1991
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 254 (5037) , 1497-1500
- https://doi.org/10.1126/science.1962210
Abstract
A polyamide nucleic acid (PNA) was designed by detaching the deoxyribose phosphate backbone of DNA in a computer model and replacing it with an achiral polyamide backbone. On the basis of this model, oligomers consisting of thymine-linked aminoethylglycyl units were prepared. These oligomers recognize their complementary target in double-stranded DNA by strand displacement. The displacement is made possible by the extraordinarily high stability of the PNA-DNA hybrids. The results show that the backbone of DNA can be replaced by a polyamide, with the resulting oligomer retaining base-specific hybridization.Keywords
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