Handling Avian Chromosomes

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Abstract
The somatic and meiotic chromosomes of Galius gal-lus dom. (as well as of the rat, mouse and possibly other animals) may be adequately prepared for miscroscopic examination by the following smear technic: Pith the fowl of appropriate age through the palatal fissure (other killing technics may be substituted for other animals), remove testes, slice medianly into halves (or quarters if large) and fix in mixture of 2 parts absolute ethanol, 1 part propionic acid, 1 part chloroform. Pour off and replace fixative at the end of 12-24 hours, after which the tissue may be stained or stored under refrigeration. Fresh material can be stained in propionic-carmine (0.5 g. carmine boiled in 45% aqueous solution of propionic acid), but material stored for several months should be stained in a boiled mixture of 10 ml. vinyl acetate, 10 ml. tertiary butyl alcohol, 50 ml. propionic acid, 75 ml. H2O, 0.25 g. carmine, 0.25 g. orcein. A method is described for preparing and making permanent such preparations.
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