Low iron concentration and aconitase deficiency in a yeast frataxin homologue deficient strain
Open Access
- 4 August 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 456 (2) , 281-284
- https://doi.org/10.1016/s0014-5793(99)00961-8
Abstract
Deletion of the yeast frataxin homologue, YFH1, elicits accumulation of iron in mitochondria and mitochondrial defects. We report here that in the presence of an iron chelator in the culture medium, the concentration of iron in mitochondria is the same in wild‐type and YFH1 deletant strains. Under these conditions, the activity of the respiratory complexes is restored. However, the activity of the mitochondrial aconitase, a 4Fe‐4S cluster‐containing protein, remains low. The frataxin family bears homology to a bacterial protein family which confers resistance to tellurium, a metal closely related to sulfur. Yfh1p might control the synthesis of iron‐sulfur clusters in mitochondria.Keywords
This publication has 25 references indexed in Scilit:
- Suppressors of Superoxide Dismutase (SOD1) Deficiency in Saccharomyces cerevisiaePublished by Elsevier ,1998
- Aconitase and mitochondrial iron–sulphur protein deficiency in Friedreich ataxiaNature Genetics, 1997
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- Respiratory deficiency due to loss of mitochondrial DNA in yeast lacking the frataxin homologueNature Genetics, 1997
- Studies of human, mouse and yeast homologues indicate a mitochondrial function for frataxinNature Genetics, 1997
- Regulation of Mitochondrial Iron Accumulation by Yfh1p, a Putative Homolog of FrataxinScience, 1997
- Friedreich's ataxia protein: phylogenetic evidence for mitochondrial dysfunctionTrends in Neurosciences, 1996
- Studies on the Synthesis of the Fe-S Cluster of Dihydroxy-acid Dehydratase in Escherichia coli Crude ExtractPublished by Elsevier ,1996
- Iron-sulfur clusters as biosensors of oxidants and ironTrends in Biochemical Sciences, 1996
- Mitochondrial iron not bound in heme and iron-sulfur centers. Estimation, compartmentation and redox stateBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1980