Prenatal Diagnosis of Hemoglobinopathies: A Potential Application of High Performance Liquid Chromatography
- 1 January 1987
- journal article
- research article
- Published by Taylor & Francis in Hemoglobin
- Vol. 11 (4) , 341-352
- https://doi.org/10.3109/03630268709042853
Abstract
High performance liquid chromatography was compared with carboxymethyl cellulose column chromatography data for analysis of globin chain composition and biosynthetic ratios in fetal blood. A formula was derived for mathematical correction for losses of small amounts of globin protein on the high-performance liquid chromatography column. Radioactive globin chain ratios by high performance liquid chromatography are equivalent to those by carboxymethyl cellulose column chromatography, but only when carrier Hb A is added. We conclude that high performance liquid chromatography provides a suitable alternative to carboxymethyl cellulose columns, and has several advantages.This publication has 14 references indexed in Scilit:
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