Microdetermination of Formic Acid in Periodate Oxidation Mixtures***

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Abstract
An enzymatic method is described for the estimation of formic acid formed during the periodate oxidation of carbohydrates in the 10−2 μmole range. The method is based on the coupled action of formic acid dehydrogenase and nitrate reductase obtained as a particulate fraction from Escherichia coli strains. The enzymes catalyze the quantitative reduction of nitrate with formic acid to yield nitrite. The nitrite is estimated by a photometric method. The results obtained using this method on D-glucose, L-rhamnose, N-acetyl-D-glucos-amine and UDP-glucose are given.