High-performance Liquid Chromatographic Determination of Some Amino Acids After Derivatization With 2-Hydroxy-1-naphthaldehyde
- 1 January 1997
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 122 (2) , 147-150
- https://doi.org/10.1039/a604847a
Abstract
2-Hydroxy-1-naphthaldehyde (HNA) was investigated as a derivatizing reagent for the fluorescence detection of histidine, methionine and tryptophan at 385 and 265 nm. The separation of HNA-derivatized amino acids on a conventional reversed-phase column was achieved in less than 40 min. This method was compared with the reported o-phthaldialdehyde (OPA) method. The procedure was successfully applied to the assay of the pure amino acids in a mixture and their pharmaceutical dosage forms.Keywords
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