Regulation of the Saccharomyces cerevisiae DNA repair gene RAD16
- 25 May 1995
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 23 (10) , 1679-1685
- https://doi.org/10.1093/nar/23.10.1679
Abstract
The RAD16 gene product has been shown to be essential for the repair of the silenced mating type loci [Bang et al. (1992) Nucleic Acids Res. 20, 3925-3931]. More recently we demonstrated that the RAD16 and RAD7 proteins are also required for repair of non-transcribed strands of active genes in Saccharomyces cerevisiae [Waters et al. (1993) Mol. Gen. Genet. 239, 28-32]. We have studied the regulation of the RAD16 gene and found that the RAD16 transcript levels increased up to 7-fold upon UV irradiation. Heat shock at 42 degrees C also results in elevated levels of RAD16 mRNA. In sporulating MAT alpha/MATa diploid cells RAD16 mRNA is also induced. The basal level of the RAD16 transcript is constant during the mitotic cell cycle. G1-arrested cells show normal induction of RAD16 mRNA upon UV irradiation demonstrating that the induction is not a secondary consequence of G2 cell cycle arrest following UV irradiation. However, in cells arrested in G1 the induction of RAD16 mRNA after UV irradiation is not followed by a rapid decline as occurs in normal growing cells suggesting that the down regulation of RAD16 transcription is dependent on progression into the cell cycle.Keywords
This publication has 41 references indexed in Scilit:
- The RAD7 and RAD16 genes, which are essential for pyrimidine dimer removal from the silent mating type loci, are also required for repair of the nontranscribed strand of an active gene in Saccharomyces cerevisiae.Molecular and Cellular Biology, 1994
- RAD9-dependent G1 arrest defines a second checkpoint for damaged DNA in the cell cycle of Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences, 1993
- Cloning and characterisation of the Schizosaccharomyces pombe rad8 gene, a member of the SNF2 helicase familyNucleic Acids Research, 1993
- ERCC6, a member of a subfamily of putative helicases, is involved in Cockayne's syndrome and preferential repair of active genesPublished by Elsevier ,1992
- A damage-responsive DNA binding protein regulates transcription of the yeast DNA repair gene PHR1.Proceedings of the National Academy of Sciences, 1991
- Chromosome tangling and breakage at anaphase result from mutations in lodestar, a Drosophila gene encoding a putative nucleoside triphosphate-binding protein.Genes & Development, 1991
- The RAD9 Gene Controls the Cell Cycle Response to DNA Damage in Saccharomyces cerevisiaeScience, 1988
- The yeast polyubiquitin gene is essential for resistance to high temperatures, starvation, and other stressesCell, 1987
- INDUCIBLE DNA REPAIR SYSTEMSAnnual Review of Biochemistry, 1985
- The SOS regulatory system of Escherichia coliCell, 1982