ACTIVITY OF SOIL BACTERIA ON PETROLEUM WASTE ADJACENT TO AN ACTIVE OIL WELL

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Abstract
Activity of 4 soil bacterial isolates from soils adjacent to an active oil well located in north central Louisiana [USA] was determined. Viable cell counts, shake-culture growths, gaseous evolution in soil samples inpregnated with petroleum waste, and manometric experiments on O2 uptake with selected hydrocarbons were used as measurements of activity of the soil bacteria on the petroleum waste. Of 10 primary isolates, 4 were highly active on petroleum waste and were identified as species of Pseudomonas, Alcaligenes and Achromobacter. All 4 isolates utilized the petroleum waste at high rates in a shake-culture system. Studies on gaseous evolution showed a constant rate of activity as measured by evolved CO2. Manometric studies showed a slow to moderate rate of O2 uptake on 3 selected hydrocarbons.

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