A Nomograph for Calculating the Optimal Frequency for Dielectrophoresis and the Characteristic Frequency of the β-Dispersion of Cell Membrane Vesicles

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Abstract
The first stage of the two-stage cell electrofusion technique involves the dielectrophoretic apposition, in an AC field, or protoplasts suspended in a medium of relatively low specific conductivity. A frequency at which the maximum dielectrophoretic force is exerted is given by the characteristic frequency for the dielectric relaxation by a Maxwell-Wagner type of mechanism. We provide a nomograph for the rapid calculation of this frequency.

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