Molecular cloning and sequencing of human cDNA for phosphoribosyl pyrophosphate synthetase subunit II
Open Access
- 13 February 1989
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 244 (1) , 47-50
- https://doi.org/10.1016/0014-5793(89)81159-7
Abstract
CDNA clones for human phosphoribosyl pyrophosphate synthetase subunit II (PRS II) were isolated. The five overlapping clones contained 2457 base pairs (bp) covering a 954‐bp complete coding region for 318 amino acid residues. Homologies between human and rats PRS II were 99% of the amino acid and 88% of the nucleotides in the coding region. This amino acid homology seems to be the highest so far reported for enzymes involved in nucleotide metabolism and glycolysis. The highly conserved structure may be required for unique catalysis and rigid regulation of this enzyme.Keywords
This publication has 9 references indexed in Scilit:
- Nucleotide and deduced amino acid sequences of two distinct cDNAs for rat phosphoribosylpyrophosphate synthetase.Journal of Biological Chemistry, 1987
- Superactivity of human phosphoribosyl pyrophosphate synthetase due to altered regulation by nucleotide inhibitors and inorganic phosphateBiochimica et Biophysica Acta (BBA) - General Subjects, 1986
- Phosphoribosylpyrophosphate synthetase of Escherichia coli. Properties of the purified enzyme and primary structure of the prs gene.Journal of Biological Chemistry, 1986
- Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomesCell, 1986
- Molecular evolution of mRNA: A method for estimating evolutionary rates of synonymous and amino acid substitutions from homologous nucleotide sequences and its applicationJournal of Molecular Evolution, 1980
- Synthesis of Phosphoribosylpyrophosphate in Mammalian CellsPublished by Wiley ,1979
- Evidence for X-Linkage of Phosphoribosylpyrophosphate Synthetase in ManHuman Heredity, 1977
- Human Phosphoribosylpyrophosphate Synthetase: Increased Enzyme Specific Activity in a Family with Gout and Excessive Purine SynthesisProceedings of the National Academy of Sciences, 1973
- Human Phosphoribosylpyrophosphate SynthetasePublished by Elsevier ,1971