CHARACTERIZATION OF MOUSE FETAL LIVER GRANULOCYTE-MACROPHAGE COLONY-FORMING CELLS USING VELOCITY SEDIMENTATION

  • 1 January 1978
    • journal article
    • research article
    • Vol. 6  (2) , 246-256
Abstract
Fetal liver cells from CBA mice were separated by velocity sedimentation to determine the distribution and properties of granulocyte-macrophage colony-forming cells (GM-CFC). Twelve-day fetal liver GM-CFC separated into 2 peaks (s = 7.7 mm/h, s = 9.4 mm/h) in cultures stimulated by mouse lung conditioned medium (GM-CSFMLCM) or human urine (GM-CSFHU). Because fetal liver GM-CFC are of relatively light bouyant density, fetal liver GM-CFC are apparently much larger than corresponding cells in adult bone marrow (s = 4.5 mm/h). By 18 days gestation more slowly sedimenting colony-forming cells were present (s = 4.7 mm/h, s = 6.0 mm/h). At all gestational ages, the most rapidly sedimenting (larger) colony-forming cells were more responsive to stimulation by GM-CSFMLCM than the smaller (slowly sedimenting) cells. Unlike the situation with adult marrow cells, velocity sedimentation achieved no segregation of GM-CFC according to the morphological type of colony produced.