The plastid clpP1 protease gene is essential for plant development
- 1 September 2003
- journal article
- Published by Springer Nature in Nature
- Vol. 425 (6953) , 86-89
- https://doi.org/10.1038/nature01909
Abstract
Plastids of higher plants are semi-autonomous cellular organelles that have their own genome and transcription-translation machinery. Examples of plastid functions are photosynthesis and biosynthesis of starch, amino acids, lipids and pigments. Plastid functions are encoded in approximately 120 plastid genes and approximately 3,000 nuclear genes. Although many embryo and seedling lethal nuclear genes are required for chloroplast biogenesis, until now deletion of plastid genes either had no phenotypic consequence (8 genes), or caused a mutant phenotype but did not affect viability (13 genes). Here we identify an essential plastid gene. By using the CRE-lox site-specific recombination system we have deleted clpP1 (caseinolytic protease P1), one of the three genes (clpP1, ycf1 and ycf2) whose disruption had previously only been possible in a fraction of the 1,000-10,000 plastid genome copies in a cell. Loss of the clpP1 gene product, the ClpP1 protease subunit, results in ablation of the shoot system of tobacco plants, suggesting that ClpP1-mediated protein degradation is essential for shoot development.Keywords
This publication has 29 references indexed in Scilit:
- Effects of selective inactivation of individual genes for low-molecular-mass subunits on the assembly of photosystem II, as revealed by chloroplast transformation: the psbEFLJ operon in Nicotiana tabacumMolecular Genetics and Genomics, 2003
- PCR analysis of pulsed-field gel electrophoresis-purified plastid DNA, a sensitive tool to judge the hetero-/homoplastomic status of plastid transformantsCurrent Genetics, 2003
- Chloroplast research in the genomic ageTrends in Genetics, 2003
- Evolutionary analysis ofArabidopsis, cyanobacterial, and chloroplast genomes reveals plastid phylogeny and thousands of cyanobacterial genes in the nucleusProceedings of the National Academy of Sciences, 2002
- Lack of the Small Plastid-encoded PsbJ Polypeptide Results in a Defective Water-splitting Apparatus of Photosystem II, Reduced Photosystem I Levels, and Hypersensitivity to LightJournal of Biological Chemistry, 2002
- Chloroplast biogenesis and function are first in the list of essential Arabidopsis genes.Trends in Plant Science, 2002
- Extranuclear Inheritance: Functional Genomics in ChloroplastsPublished by Springer Nature ,2002
- Arabidopsis Genes Essential for Seedling Viability: Isolation of Insertional Mutants and Molecular CloningGenetics, 2001
- Efficient elimination of selectable marker genes from the plastid genome by the CRE‐lox site‐specific recombination systemThe Plant Journal, 2001
- The chloroplast genomePlant Molecular Biology, 1992