“Bound” Insulin: Biologic Effects in Intact, Hypophysectomized or Adrenalectomized Rats Following Intravenous Administration

Abstract
"Bound" insulin concentrates, obtained from pooled human sera, or crystalline insulin standards were injected intravenously into intact, hypophysectomized or adrenal-ectomized rats. Both "bound" and crystalline insulin, in the amounts used, caused a significant decline in the blood glucose concentrations of the hypophysectomized or adrenalectomized rats without affecting the blood glucose concentration of the intact rats. Administration of "bound" or crystalline insulin, along with trace amount of glucose -[mu]-C14 stimulated the incorporation of glucose carbon into the glycogen of the diaphragm and into the fat of the adipose tissue of hypophysectomized and adrenalectomized rats. The effects of "bound" insulin in these studies were similar to those of crystalline insulin.