Two forms of simian-virus-40-specific T-antigen in abortive and lytic infection.

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Abstract
SV-40-specific T[tumor]-antigen was isolated by immunoprecipitation. The T-antigen described in this work is apparently coded by the viral DNA. The MW estimated from electrophoretic mobility in sodium dodecyl sulfate-polyacrylamide gels of T-antigen isolated from nonpermissive mouse [primary kidney] cells in abortive infection is 86,000, and from permissive [African green] monkey [kidney Vero, CV-1 and BSC] cells [and primary vervet kidney cells] in lytic infection is 82,000. The 86 kilodalton T-antigen is readily converted in vitro into an 82 kilodalton form by incubation with extracts from permissive monkey cells but not with extracts from nonpermissive mouse or hamster [kidney BHK] cells. This and the results of fingerprinting analysis of tryptic peptides suggest that T-antigen may be processed in permissive cells.