Accumulation and Subcellular Localization of α-Galactosidase-Hemagglutinin in Developing Soybean Cotyledons
Open Access
- 1 April 1985
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 77 (4) , 886-890
- https://doi.org/10.1104/pp.77.4.886
Abstract
We have investigated the accumulation and intracellular localization of soybean (Glycine max [L.] Merr. cv Forrest) α-galactosidase-hemagglutinin during seed development. Cotyledon tissue was embedded in Lowicryl K4M and immunocytochemical localization was accomplished through treating thin sections with α-galactosidase antisera followed by indirect labeling with protein A coupled to colloidal gold. Gold particles were localized on the Golgi apparatus and protein bodies. We interpret this to indicate that α-galactosidase-hemagglutinin is transferred to and transported through the Golgi apparatus and finally deposited within the protein body by a Golgi apparatus-mediated process.This publication has 25 references indexed in Scilit:
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