COMPARATIVE ULTRASTRUCTURAL EFFECTS OF AFLATOXIN B-1 ON MOUSE, RAT, AND HUMAN HEPATOCYTES IN PRIMARY CULTURE
- 1 March 1986
- journal article
- research article
- Vol. 46 (3) , 1290-1296
Abstract
Human, rat, and mouse hepatocytes in primary culture were treated with aflatoxin B1 (AFB1) and examined for ultrastructural alterations. As early as 1 h following in vitro exposure to AFB1, there were ultrastructural changes in the nuclei of rat and human hepatocytes. The most prominent change in the nuclei was a segregation of nucleolar components that resembled the segregation in liver cells of rats exposed to AFB1 in vivo. The nucleolar segregations were developed by incubating rat hepatocytes for 24 h in a medium containing as little as 0.01 .mu.g of AFB1 per ml. The minimum concentration to induce the same change in human hepatocytes was 0.1 .mu.g/ml. No distinct nucleolar alteration was observed in mouse hepatocytes incubated in a medium containing 10 .mu.g of AFB1 per ml. Irregular nuclear chromatin condensation also developed in the cells exposed to a higher concentration of AFB1, whereas little damage was observed in mitochondria and lysosomes. The similarity in morphological changes between our in vitro model and in vivo models previously investigated indicates that the hepatocytes in primary culture maintain the biological properties necessary for carcinogen responses similar to liver cells in vivo. In addition, the morphological changes in cultured rat and mouse hepatocytes induced by AFB1 correlate with in vivo experiments insofar as mice are relatively resistant, whereas rats are sensitive to AFB1 carcinogenesis. Thus, cultured hepatocyte systems may be a valuable tool to study genetic damage which may lead to hepatocellular carcinomas in human and animal livers.This publication has 9 references indexed in Scilit:
- EFFECTS OF AMSACRINE AND OTHER DNA-INTERCALATING DRUGS ON NUCLEAR AND NUCLEOLAR STRUCTURE IN CULTURED V79 CHINESE-HAMSTER CELLS AND PTK2 RAT KANGAROO CELLS1985
- Metabolic basis of the species difference to aflatoxin B1 induced hepatotoxicityBiochemical and Biophysical Research Communications, 1983
- LINEAR DOSE-RESPONSE CURVE FOR THE HEPATIC MACROMOLECULAR BINDING OF AFLATOXIN-B1 IN RATS AT VERY LOW EXPOSURES1982
- Differences in aflatoxin B1-susceptibility of rat and mouse are correlated with the capability in vitro to inactivate aflatoxin B1- epoxideCarcinogenesis: Integrative Cancer Research, 1981
- LIGHT AND ELECTRON MICROSCOPIC RADIOAUTOGRAPHY OF HEPATIC CELL NUCLEOLI IN MICE TREATED WITH ACTINOMYCIN DThe Journal of cell biology, 1967
- Variations in ultrastructural nuclear changes in hepatocarcinogenesisBiochemical Pharmacology, 1967
- COMPARATIVE STUDIES OF NUCLEOLI OF MORRIS HEPATOMAS EMBRYONIC LIVER AND AFLATOXIN B1-TREATED LIVER OF RATS1967
- SPECIFIC NUCLEAR AND NUCLEOLAR ULTRASTRUCTURAL LESIONS INDUCED BY PROFLAVIN AND SIMILARLY ACTING ANTIMETABOLITES IN TISSUE CULTURE1966
- AFLATOXIN B1 INJURY IN RAT AND MONKEY LIVER1966