Purification, crystallisation and preliminary X‐ray analysis of the vanadium‐dependent haloperoxidase from Corallina officinalis
- 13 February 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 359 (2-3) , 244-246
- https://doi.org/10.1016/0014-5793(95)00055-e
Abstract
The vanadium-dependent haloperoxidase from the seaweed Corallina officinalis has been purified to homogeneity and crystallised. The protein is reported to be a hexamer of 12 × 64,000 Da, contains no haem, and is dependent on vanadium for activity. The crystals are grown from polyethylene glycol (PEG) 6,000 and 0.4 M potassium chloride. They are stable and diffract to better than 2 Å resolution. They are of a cubic space group I23 (or I2,3) with cell dimensions a = b = c = 310 ÅKeywords
This publication has 16 references indexed in Scilit:
- The chloroperoxidase from the fungus Curvularia inaequalis; a novel vanadium enzymePublished by Elsevier ,2003
- The involvement of hydrogen peroxide in the production of volatile halogenated compounds by Meristiella gelidiumPhytochemistry, 1994
- Purification and characterization of the vanadium bromoperoxidase from the macroalga Corallina officinalisPhytochemistry, 1992
- Vanadium K-edge x-ray absorption spectroscopy of bromoperoxidase from Ascophyllum nodosumBiochemistry, 1989
- Vanadate activation of bromoperoxidase from CorallinaofficinalisBiochemical and Biophysical Research Communications, 1989
- The first crystallization of a vanadium‐dependent peroxidaseFEBS Letters, 1988
- Vanadium containing bromoperoxidase: An example of an oxidoreductase with high operational stability in aqueous and organic mediaBiotechnology & Bioengineering, 1987
- The bromoperoxidase from the red alga Ceramium rubrum also contains vanadium as a prosthetic groupBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1987
- Isolation and characterization of a novel nonheme chloroperoxidaseBiochemical and Biophysical Research Communications, 1987
- Maturation of the head of bacteriophage T4Journal of Molecular Biology, 1973