Serologic Differentiation of Trachoma Strains and Other Agents of the Psittacosis - Lymphogranuloma Venereum-Trachoma Group with the Aid of the Direct Fluorescent Antibody Method

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Abstract
Summary: The serologic relationship of various strains of the psittacosis-lymphogranuloma venereum-trachoma group of agents was examined with the aid of rabbit immune sera and antigens partially purified with fluorocarbon or diethylaminoethyl cellulose. The direct fluorescent antibody technique was employed. Major sharing of antigens between trachoma and inclusion conjuctivitis (TRIC) agents and two strains of lymphogranuloma venereum was demonstrated. No cross-reactions were observed between these strains and a strain of psittacosis. By the use of cross-absorbed immune sera, a subdivision of TRIC agents was obtained according to which strains Dari and Camal, isolated in Israel, and strains Bour and T'ang fell in Group I and strains Asgh, SA-1 and G-17 in Group II. The strain of inclusion conjunctivitis IC Cal-3 showed cross-reactions with both groups of trachoma and, in addition, some specificity of its own. With the aid of cross-absorbed rabbit immune sera it was possible to group trachoma agents directly from infected yolk sac smears.