Retardation of Sodium Exchange in Dog Erythrocytes by Physiological Concentrations of Aldosterone, in Vitro*

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Abstract
A log dose-response relationship has been found between the concentration of D-aldosterone added to dog blood incubated in vitro, at 37[degree] C, in 95% O2-5% CO2 with added glucose and Na22Cl, and the rate of Na influx into the erythrocytes. When blood was used from 2 adrenalectomized dogs, treated with cortisol but without mineralo-corticoids, Na flux (22.30[plus or minus]1.90 mEq/L. cells/hour) was significantly higher than in the blood of the same dog before the adrenalectomy (13.88[plus or minus] 0.42, p .01) and was progressively reduced to 5.5 mEq/L cells/hour by concentrations of added D-aldosterone between 0.24 and 1.99 ug./L. plasma, without further reduction of Na influx at D-aldosterone concentrations of up to 23.1 ug./L.