Generation of a floxed allele of Smad5 for cre‐mediated conditional knockout in the mouse
- 29 August 2003
- Vol. 37 (1) , 5-11
- https://doi.org/10.1002/gene.10219
Abstract
Summary: Smad5 is a member of the Smad family of intracellular mediators of BMP signals and in endothelial cells of TGF-β signals. We and others previously showed that loss of Smad5 in the mouse results in embryonic lethality (between E9.5–E11.5) due to multiple embryonic and extraembryonic defects. To circumvent the early embryonic lethality and to allow tissue- and time-specific Smad5 inactivation, we created a conditional Smad5 allele in the mouse. Floxed Smad5 (Smad5flE2,Neo/flE2,Neo) mice were generated in which both exon2 and the Neo-cassette were flanked by loxP sites. Here we demonstrate that embryos with ubiquitous Cre-mediated deletion of Smad5 (Smad5flΔE2/flΔE2) phenocopy the conventional Smad5 knockout mice. Smad5flE2/flE2 mice are now available and will be a valuable tool to analyze the role of Smad5 beyond its crucial early embryonic function throughout development and postnatal life. genesis 37:5–11, 2003.Keywords
This publication has 27 references indexed in Scilit:
- Disruption of Smad5 gene leads to enhanced proliferation of high-proliferative potential precursors during embryonic hematopoiesisBlood, 2003
- Activin receptor–like kinase 1 is implicated in the maturation phase of angiogenesisBlood, 2002
- Requirement of Bmpr1a for Müllerian duct regression during male sexual developmentNature Genetics, 2002
- Regulation of cell proliferation by Smad proteinsJournal of Cellular Physiology, 2002
- Transforming growth factor β signalling in vitro and in vivo: activin ligand–receptor interaction, Smad5 in vasculogenesis, and repression of target genes by the δEF1/ZEB-related SIP1 in the vertebrate embryoMolecular and Cellular Endocrinology, 2001
- Mullerian Inhibiting Substance Signaling Uses a Bone Morphogenetic Protein (BMP)-Like Pathway Mediated by ALK2 and Induces Smad6 ExpressionMolecular Endocrinology, 2001
- Remarkable versatility of Smad proteins in the nucleus of transforming growth factor-β activated cellsCytokine & Growth Factor Reviews, 1999
- Requirement of the transcription factor GATA4 for heart tube formation and ventral morphogenesis.Genes & Development, 1997
- The C-terminal domain of Mad-like signal transducers is sufficient for biological activity in the Xenopus embryo and transcriptional activationMechanisms of Development, 1997
- Efficient in vivo manipulation of mouse genomic sequences at the zygote stage.Proceedings of the National Academy of Sciences, 1996