RADIOIMMUNOASSAY OF ATROPINE IN PLASMA

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 203  (2) , 435-441
Abstract
Antibodies, against atropine, were produced in rabbits immunized with atropine conjugated to bovine serum albumin. The antisera possessed a high binding affinity and were quite specific. The sensitivity of the method allowed detection of 6.25 ng of atropine/ml of plasma in a 10-.mu.l specimen. The method did not require an extraction procedure and can be performed using very small plasma volumes. Plasma concentration-time profiles were determined by this method in dogs after rapid i.v. administration of atropine. Atropine declined from plasma in a biexponential fashion, exhibiting a terminal half-life of approximately 2.1 h.

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