High pressure reverse phase liquid chromatography of fatty acidp‐bromophenacyl esters

Abstract
High pressure reverse phase liquid chromatography has been employed to rapidly separate saturated and unsaturated fatty acids as the correspondingp-bromophenacyl esters. Through the use of a highly efficient C18 reverse phase column packing, it has also been possible to distinguish among geometrical and positional isomers of the unsaturated acids. The use of ultroviolet-sensitive esters has permitted the detection of low (nanogram range) concentrations of fatty acids. The time required for analysis has been further reduced by employing a novel and rapid method for the preparation of the esters.