THE CONVERSION OF PREGNENOLONE-7-3H AND PROGESTERONE-4-14C TO OESTRADIOL BY A CORPUS LUTEUM OF PREGNANCY

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Abstract
The conversion by a corpus luteum of pregnancy of pregnenolone-7-H3 and progesterone-4-C14 to estradiol in 1% yield has been reported. The estradiol was characterized by solvent partition, chromatography, enzymatic conversion to estrone, methyl ether formation and crystallization after carrier addition. A similar ratio of H3 to C14 was found in the starting materials, in the 17-hydroxyprogesterone intermediate and in the end product estradiol. No evidence was found for a significant alternate pathway of estrogen formation via [DELTA]5-3[beta] ol intermediates in this tissue.