Application of the Biological Conjugate between Antibody and Colloid Au Nanoparticles as Analyte to Inductively Coupled Plasma Mass Spectrometry

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Abstract
This paper describes the study of atomization of nanoparticles by inductively coupled plasma mass spectrometry (ICPMS) and developes a novel nonisotopic immunoassay by coupling sandwich-type immunoreaction to ICPMS. The goat-anti-rabbit immunoglobulin G (IgG) labeled with colloidal gold nanoparticles served as an analyte in ICPMS for the indirect measurement of rabbit-anti-human IgG. Matrix effect studies showed the gold signal was not sensitive to the organic matrix. A relatively good correlation (r2 = 0.9528) between the proposed method and enzyme-linked immunosorbent assay has been obtained. The method may have significant potential as an important ICPMS-based nonisotopic immnoassay method for the simultaneous determination of biologic analytes of interest by labeling different kinds of inorganic nanoparticles.