Inactivation by Electron Radiation of the Capacity of Goldenrod Polysaccharide to Induce Vacuolization in Ascites Tumor Cells

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Abstract
A polysaccharide from goldenrod was exposed to various doses of 7 kv electrons from a tungsten filament. The attenuation of the capacity of this polymer to induce cytoplasmic vacuolization and enlargement in Sarcoma 37 ascites tumor cells was studied. The logarithm of survival for this property was found to be a nearly linear function of electron dose, within the limits of experimental error. No negative curvature near zero dose was observed. Dose-survival curves for the inactivation of the enzymic functions of ribonuclease (mol. wt. 13,700) and invertase (mol. wt. 120,000) were obtained in the same manner. A comparison with these curves leads to the conclusion that the molecular weight of the polysaccharide moiety responsible for cell vacuolization is in the order of 8,000.