Ceftazidime and Aztreonam Resistance in Providencia stuartii : Characterization of a Natural TEM-Derived ExtendedSpectrum β-Lactamase, TEM-60
- 1 June 1998
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 42 (6) , 1459-1462
- https://doi.org/10.1128/aac.42.6.1459
Abstract
A plasmid-encoded β-lactamase produced from a clinical strain of Providencia stuartii has been purified and characterized. The gene coding for the β-lactamase was cloned and sequenced. It appears to be a new natural TEM-derived enzyme, named TEM-60. Point mutations (Q39K, L51P, E104K, and R164S) are present with respect to the TEM-1 enzyme; the mutation L51P has never been previously reported, with the exception of the chromosomally encoded extended-spectrum β-lactamase PER-1. Kinetic parameters relative to penicillins, cephalosporins, and monobactams other than mechanism-based inactivators were related to the in vitro susceptibility phenotype.Keywords
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