Histone Adduction with Nicotine: A Bio-Ams Study
Open Access
- 1 January 1997
- journal article
- research article
- Published by Cambridge University Press (CUP) in Radiocarbon
- Vol. 39 (3) , 293-297
- https://doi.org/10.1017/s0033822200053273
Abstract
Based on the study of DNA adduction with nicotine, we have measured the mouse hepatic histone adduction with14C-labeled nicotinein vivoby bio-accelerator mass spectrometry (bio-AMS). In the exposure of mice to nicotine, the dose range administered was from 0.2 μg to 6.0 μg kg b.w.-1, which was equivalent to a very low level of human exposure to cigarette smoke. The adducts of either histone 1 (H1) or histone 3 (H3) with nicotine in mouse liver increased markedly with increasing nicotine dose. Our results have demonstrated that in the study of protein adduction with toxic xenobiotics as a biomarker, the AMS method achieves the highest sensitivity, 4.6 × 10-17mol (46 amol) adducts per mg H1 protein, compared to all the other methods used previously.Keywords
This publication has 19 references indexed in Scilit:
- AMS Adds Realism to Chemical Risk AssessmentScience, 1996
- Tobacco‐specificN‐nitrosamines andARECA‐derivedN‐nitrosamines: Chemistry, biochemistry, carcinogenicity, and relevance to humansJournal of Toxicology and Environmental Health, 1994
- Significance of DNA adduct studies in animal models for cancer molecular dosimetry and risk assessment.Environmental Health Perspectives, 1993
- Use of biomarkers in epidemiology: quantitative aspectsToxicology Letters, 1992
- Cambrian JoltScientific American, 1992
- Histones as Regulators of GenesScientific American, 1992
- Accelerator mass spectrometry in the biomedical sciences: applications in low-exposure biomedical and environmental dosimetryNuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 1990
- Status of the tandem accelerator mass spectrometry facility at Peking UniversityNuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 1990
- Effects of eluent composition, ion-pair reagent and temperature on the separation of histones by high performance liquid chromatographyChromatographia, 1990
- Reactivity and adduct formation of a polyaromatic hydrocarbon, 7-bromomethylbenz[a]anthracene, with chromatin histone proteinsJournal of Chromatography B: Biomedical Sciences and Applications, 1990