Comparative Study of Procedures for Quantification of Vibrio parahaemolyticus in Seafoods

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Abstract
A collaborative study was made comparing the efficacy of combinations of two broths and two agar media for recovery and quantification of Vibrio parahaemolyticus and Vibrio alginolyticus. Broths used in the study were Glucose Salt Teepol broth (GSTB) and Horie broth (HB); agars were Thiosulfate-Citrate-Bile Salts-Sucrose agar (TCBS) and water blue-alizarin-yellow agar (W B). Frozen shrimp samples seeded with high, medium and low concentrations of V. parahaemolyticus and medium concentrations of V. alginolyticus were used in this study. Results showed that in general all media using HB gave M PN values 10 times greater than combinations employing GSTB. The HB-WB combination gave V. parahaemolyticus recoveries 10-fold higher than the other media combinations tested.

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