SOME CYTOFLUORIMETRIC STUDIES OF THE NUCLEAR PLOIDY OF MOUSE HEPATOCYTES .1. A SIMPLE METHOD FOR ISOLATION OF HEPATOCYTE NUCLEI USING INSITU PERFUSION OF THE LIVER
- 1 January 1981
- journal article
- research article
- Vol. 62 (5) , 469-473
Abstract
A simple method for isolating mouse hepatocyte nuclei is presented, which requires no special apparatus. Flow cytofluorimetric scans of hepatocyte nuclei, prepared by this method and stained with ethidium bromide, showed adult mice have a high proportion of their hepatocyte nuclei in the tetraploid and octaploid states. Polyploidy was more pronounced in female animals.This publication has 3 references indexed in Scilit:
- Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining.The Journal of cell biology, 1975
- Cell Microfluorometry: A Method for Rapid Fluorescence MeasurementScience, 1969
- CELL SIZE, NUCLEAR CONTENT, AND THE DEVELOPMENT OF POLYPLOIDY IN THE MAMMALIAN LIVERProceedings of the National Academy of Sciences, 1967