More on p53 Antigen Loss in Stored Paraffin Slides

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Abstract
Prioleau and Schnitt (June 1 issue)1 reported a potential problem of p53 antigen loss in slides that had been stored at room temperature for two months. We examined slides that had been cut from formalin-fixed, paraffin-embedded blocks containing a p53-positive colorectal carcinoma and accidentally stored at room temperature for periods of 6 to 14 months. We found that the p53 reactivity could be restored with a method of antigen retrieval2 based on heating the slides in a microwave oven.