Ultraviolet Spectrophotometric Analysis of Barbiturates: Evaluation of Potential Interferences
Open Access
- 1 February 1973
- journal article
- research article
- Published by Oxford University Press (OUP) in American Journal of Clinical Pathology
- Vol. 59 (2) , 167-173
- https://doi.org/10.1093/ajcp/59.2.167
Abstract
Twenty drugs, including salicylic acid and sulfonamides, were studied to determine whether they interfered with the ultraviolet spectrophotometric analysis of barbiturates. These were drugs which have been cited as possibly or probably interfering with this type of analysis. Only p-aminophenol, which would not be expected in blood in the free form, interfered. Ultraviolet spectrophotometry is suitable for the emergency analysis of barbiturates in the hospital laboratory. It is recommended that the method selected utilize extraction at neutral pH, and differential spectrophotometry in which equal concentrations of drugs at pH 13 and pH 10 are compared directly with a single scan. The net difference between the absorbances measured at wavelengths of 260 nm. and 240 nm. is least subject to interference and can be used for quantification. At these wavelengths interference from other drugs is rare.Keywords
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