Isolation and characterization of adult bovine heart muscle chalones.
- 1 July 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (7) , 4161-4164
- https://doi.org/10.1073/pnas.78.7.4161
Abstract
A 3-step purification procedure for the isolation of heart muscle chalones is described. The method uses ultrafiltration followed by concanavalin A-Sepharose affinity chromatography and subsequent sucrose density gradient centrifugation. Analytical ultracentrifugation of the highly purified chalone fraction indicated a sedimentation coefficient of 16.7 S and an average MW of 715,527. Chemical analyses have confirmed this protein to be a glycoprotein. Polypeptide analysis indicated the involvement of identical subunits in its composition. In vivo studies confirmed that the chalone isolated from adult bovine hearts inhibits DNA synthesis of newborn hamster hearts. It was tissue and not species specific and had no cytotoxic effects on the target cells.This publication has 14 references indexed in Scilit:
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