Induction of glyoxylate cycle enzymes in rat liver upon food starvation

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Abstract
The key enzymes of the glyoxylate cycle, isocitrate lyase and malate synthase, have been detected in liver of foodstarved rats. Activities became measurable 3 days and peaked 5 days after the beginning of starvation. Both enzymes were found in the peroxisomal cell fraction after organelle fractionation by isopycnic centrifugation. Isocitrate lyase was purified 112‐fold by ammonium sulfate precipitation, and chromotography on DEAE‐cellulose and Toyopearl HW‐65. The specific activity of the purified enzyme was 9.0 units per mg protein. The K m(isocitrate) was 68 μM and the pH optimum was at pH 7.4. Malate synthase was enriched 4‐fold by ammonium sulfate precipitation. The enzyme had a K m(acetyl‐CoA) of 0.2 μM, a K m(glyoxylate) of 3 mM and a pH optimum of 7.6.