Auxin- and Ethylene-Induced Changes in the Population of Translatable Messenger RNA in Basal Sections and Intact Soybean Hypocotyl

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Abstract
In vitro translation products of polyadenylated RNA from untreated and auxin-treated basal sections of soybean (G. max cv. Wayne) hypocotyl were analyzed by 2-dimensional polyacrylamide gel electrophoresis. Within 1 h of 2,4-D treatment, the translatable mRNA for at least 12 in vitro translation products are modulated upward. In vitro translation products of polyadenylated RNA from untreated, auxin-treated and ethephon-treated intact soybean hypocotyl were also analyzed. Within 2 h of treatment with either 2,4-D or ethephon, the translatable mRNA for a group of high-MW in vitro translation products are modulated upward. There is a particular set of translatable mRNA, encoding in vitro translation products in the 24,000-32,000 MW range, that is specifically modulated upward by auxin treatment in intact soybean hypocotyl and in hypocotyl sections.