The Measurement of the Growth of Mycoplasma in Liquid Media

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Abstract
SUMMARY: The growth of Mycoplasma laidlawii strain A in liquid media was estimated by colony count, turbidity, and by measurement of dry weight, total-N and deoxyribonucleic acid (DNA) of washed deposits from centrifuged cultures. Samples from growing cultures were also examined with the phase-contrast microscope. The growth curves of cultures obtained by the colony count method resembled a bacterial growth curve. The other methods became useful only when nearly maximum growth had occurred because of the very small yield of organism per unit volume of culture. The growth of organisms in two different media was compared; the growth curves obtained by the colony count were nearly identical but values for total-N and DNA were different.

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