Glial fibrillary acidic protein and vimentin immunohistochemistry in the developing and adult midbrain of the lizard Gallotia galloti

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Abstract
The distribution of glial fibrillary acidic protein (GFAP)-and vimentin-containing cells was studied by immunohistochemistry in the midbrain of the lizard Gallotia galloti. At embryonic stage 32 (E32), vimentin immunoreactivity appeared first in cell bodies located in the ventricular walls, in radial fibers, and subpial end-feet and increased in these structures until E34/E35. Faint GFAP immunoreactivity gradually appeared in the same structures between E34 and E37, and this increased until adulthood, whereas vimentin immunoreactivity decreased after E35, becoming limited to a few end-feet and fibers in the adult, mainly in the tegmentum. Thus, in developing Gallotia midbrain a shift from vimentin-containing to GFAP-containing intermediate filaments begins around E36 or E37. At E40, in addition to the cell bodies in the ependymal area, dispersed GFAP-positive cells, possibly immature astrocytes appeared. These cells showed the same shift. In the adult lizard, GFAP-positive radial glia are still present and coexist with GFAP-positive astrocytes, which are prefentially located in the marginal optic tract and the oculomotor nuclei, but are absent in the fasciculus longitudinalis medialis. Optic tectum, pretectum, tegmentum, and isthmic nuclei are the areas richest in GFAP-positive radial fibers: these were much less abundant in the deep mesencephalic nuclei. Thus, in this lizard, GFAP-positive astrocytes display a clear cut regional distribution: they are present in mesencephalon, whereas they are absent in telencephalon Yanes et al.: J. Comp. Neurol.,′90:295:559–568.